PA5-28406

antibody from Invitrogen Antibodies

Targeting: ELMO1 CED-12, CED12, ELMO-1, KIAA0281

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28406

Provider

Invitrogen Antibodies

Product name

ELMO1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A431, H1299, HeLa, HepG2, N2a, PC-12, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Neuronal adaptor FE65 stimulates Rac1-mediated neurite outgrowth by recruiting and activating ELMO1.

Li W, Tam KMV, Chan WWR, Koon AC, Ngo JCK, Chan HYE, Lau KF
The Journal of biological chemistry 2018 May 18;293(20):7674-7688

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ELMO1 Polyclonal Antibody (Product # PA5-28406). Sample (30 µg of whole cell lysate). Lane A: H1299. 12% SDS PAGE. ELMO1 Polyclonal Antibody (Product # PA5-28406) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELMO1 Polyclonal Antibody detects ELMO1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw 264.7 whole cell lysate/extract. G. 30 µg C2Cl2 whole cell lysate/extract.7.5% SDS-PAGE. ELMO1 Polyclonal Antibody (Product # PA5-28406) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELMO1 Polyclonal Antibody detects ELMO1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.7.5% SDS-PAGE. ELMO1 Polyclonal Antibody (Product # PA5-28406) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ELMO1 in methanol-fixed HeLa cells using an ELMO1 polyclonal antibody (Product # PA5-28406) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human stomach, using ELMO1 (Product # PA5-28406) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL