Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [12]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Other assay [2]
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Validation data
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- Product number
- 15112-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAPRIN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RQRVQDLMA QMQGPYNFIQ DSMLDFENQT LDPAIVSAQP MNPTQNMDMP QLVCPPVHSE SRLAQPNQVP VQPEATQVPL VSSTSEGYTA SQPLYQPSHA TEQRPQKEPI DQIQATISLN TDQTTASSSL PAASQPQVFQ AGTSKPLHSS GINVNAAPFQ SMQTVFNMNA PVPPVNEPET LKQQNQYQAS YNQSFSSQPH QVEQTELQQE QLQTVVGTYH GSPDQSHQVT GNHQQPPQQN TGFPRSNQPY YNSRGVSRGG SRGARGLMNG YRGPANGFRG GYDGYRPSFS NTPNSGYTQS QFSAPRDYSG YQRDGYQQNF KRGSGQSGPR GAPRGRGGPP RPNRGMPQMN TQQVN (356-709 aa encoded by BC001731)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of anti-CAPRIN1 (15112-1-AP, 1:800) with HeLa and ND7 cells by Emily Towers.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CAPRIN1 antibody 15112-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with OC2 cells by Dr. Emily Towers.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with Cochlear cryosections by Dr. Emily Towers. CAPRIN1, purple; DAPI, blue.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CAPRIN1 (IP:15112-1-AP, 4 ug; Detection:15112-1-AP 1:5000) with HeLa cells lysate 1600 ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CAPRIN1 (IP:15112-1-AP, 3ug; Detection:15112-1-AP 1:300) with HeLa cells lysate 2500ug.