Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-114941 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPIP137 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.
LaForce GR, Farr JS, Liu J, Akesson C, Gumus E, Pinkard O, Miranda HC, Johnson K, Sweet TJ, Ji P, Lin A, Coller J, Philippidou P, Wagner EJ, Schaffer AE
Neuron 2022 Apr 20;110(8):1340-1357.e7
Neuron 2022 Apr 20;110(8):1340-1357.e7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPIP137 in HeLa and ND7 cells. Samples were incubated with polyclonal antibody (Product # PA5-114941).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPIP137 in various samples (Lane 1: Rat brain treated with blocking peptide Lane 2: Rat brain; Lane 3: 3T3-L1; Lane 4: HepG2). Samples were incubated with polyclonal antibody (Product # PA5-114941).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GPIP137 in HepG2. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114941) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GPIP137 in human breast cancer tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114941) at a dilution of 1:100. Secondary staining was applied with HRP conjugated goat anti-rabbit.