TA319581

antibody from OriGene

Targeting: BIN1 AMPH2, AMPHL, SH3P9

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319581

Provider

OriGene

Product name

Mouse monoclonal anti-BIN1 antibody

Antibody type

Monoclonal

Description

Mouse monoclonal anti-BIN1 antibody

Host

Mouse

Conjugate

Unconjugated

Epitope

BIN1

Antibody clone number

2F11

Vial size

100 µg

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Mouse Anti-BIN-1 antibody. Lane 1: Keratinocyte derived from Bin-1 wild type. Lane 2: Keratinocyte derived from Bin-1 null mice. Load: 35 ug per lane. Primary antibody: BIN-1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800„? mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20 (PBS/T ) overnight at 4°C. Bin1 isoforms (+exon13, -exon13). *indicates non-specific signal.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type (+/+) and null (-/-) mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.

Validation comment

IHC