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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX100459 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100459, RRID:AB_10618561
- Product name
- MUC1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE GTX100459 diluted at 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with MUC1 antibody (GTX100459) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using MUC1(GTX100459) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MUC1 antibody detects MUC1 protein at cytoplasm by immunofluorescent analysis.Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.Green: MUC1 protein stained by MUC1 antibody (GTX100459) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of MUC1 protein from HCT-116 whole cell extracts using 5 £gg of MUC1 antibody (GTX100459).Western blot analysis was performed using MUC1 antibody (GTX100459).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MUC1 antibody detects MUC1 protein at cytoplasm in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. MUC1 antibody (GTX100459) diluted at 1:500.
