14-9893-82
antibody from Invitrogen Antibodies
Targeting: MUC1
ADMCKD, ADMCKD1, CD227, MCD, MCKD, MCKD1, PEM, PUM
Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- 14-9893-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD227 (Mucin 1) Monoclonal Antibody (SM3), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This SM3 monoclonal antibody reacts with the under-glycosylated form of human Mucin 1 (MUC1, CD227), a large glycoprotein belonging to the mucin protein family. Mucin 1 contains a polypeptide core consisting of multiple tandem repeats that become highly glycosylated. Mucin 1 is typically expressed in ductal or glandular epithelial cells and is localized to the apical membrane. In cancerous cells, Mucin 1 expression is increased and membrane-specific localization is lost resulting in expression throughout the membrane and cytoplasm. High levels of under-glycosylated Mucin 1 are thought to affect cell behavior during both invasion and metastasis as well as in immune recognition. In addition, under-glycosylated Mucin 1 is shed from the epithelial cell surface and can be detected in circulation. Alterations in Mucin 1 glycosylation are found in most adenocarcinomas of the breast, lung, pancreas, prostate, and ovary. Mucin 1 has recently been shown to co-localize and interact with members of the erbB receptor kinase family, proteins that are upregulated in more aggressive forms of breast cancer. Please note this antibody sees a distinct epitope from other Mucin 1 antibodies. Applications Reported: This SM3 antibody has been reported for use in western blotting, immunohistochemical staining, and immunocytochemistry. Applications Tested: This SM3 antibody has been tested by western blot and immunocytochemistry on MCF7 cell line as well as by immunohistochemistry on FFPE (formalin-fixed paraffin embedded) tissue. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SM3
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Patterns of MUC1 tissue expression defined by an anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer.
Immunohistochemical study of the expression of MUC5AC and MUC6 in breast carcinomas and adjacent breast tissues.
Mice with spontaneous pancreatic cancer naturally develop MUC-1-specific CTLs that eradicate tumors when adoptively transferred.
Comparison of O-linked carbohydrate chains in MUC-1 mucin from normal breast epithelial cell lines and breast carcinoma cell lines. Demonstration of simpler and fewer glycan chains in tumor cells.
Cytotoxic T cells from ovarian malignant tumors can recognize polymorphic epithelial mucin core peptides.
A short sequence, within the amino acid tandem repeat of a cancer-associated mucin, contains immunodominant epitopes.
Croce MV, Isla-Larrain MT, Rua CE, Rabassa ME, Gendler SJ, Segal-Eiras A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2003 Jun;51(6):781-8
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2003 Jun;51(6):781-8
Immunohistochemical study of the expression of MUC5AC and MUC6 in breast carcinomas and adjacent breast tissues.
Pereira MB, Dias AJ, Reis CA, Schmitt FC
Journal of clinical pathology 2001 Mar;54(3):210-3
Journal of clinical pathology 2001 Mar;54(3):210-3
Mice with spontaneous pancreatic cancer naturally develop MUC-1-specific CTLs that eradicate tumors when adoptively transferred.
Mukherjee P, Ginardi AR, Madsen CS, Sterner CJ, Adriance MC, Tevethia MJ, Gendler SJ
Journal of immunology (Baltimore, Md. : 1950) 2000 Sep 15;165(6):3451-60
Journal of immunology (Baltimore, Md. : 1950) 2000 Sep 15;165(6):3451-60
Comparison of O-linked carbohydrate chains in MUC-1 mucin from normal breast epithelial cell lines and breast carcinoma cell lines. Demonstration of simpler and fewer glycan chains in tumor cells.
Lloyd KO, Burchell J, Kudryashov V, Yin BW, Taylor-Papadimitriou J
The Journal of biological chemistry 1996 Dec 27;271(52):33325-34
The Journal of biological chemistry 1996 Dec 27;271(52):33325-34
Cytotoxic T cells from ovarian malignant tumors can recognize polymorphic epithelial mucin core peptides.
Ioannides CG, Fisk B, Jerome KR, Irimura T, Wharton JT, Finn OJ
Journal of immunology (Baltimore, Md. : 1950) 1993 Oct 1;151(7):3693-703
Journal of immunology (Baltimore, Md. : 1950) 1993 Oct 1;151(7):3693-703
A short sequence, within the amino acid tandem repeat of a cancer-associated mucin, contains immunodominant epitopes.
Burchell J, Taylor-Papadimitriou J, Boshell M, Gendler S, Duhig T
International journal of cancer 1989 Oct 15;44(4):691-6
International journal of cancer 1989 Oct 15;44(4):691-6
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma, using 5 µg/mL of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (left) or Anti-Human CD227 (Mucin 1) Purified (right) followed by Anti-Mouse Biotin, and DAB visualization.Nuclei are counterstained with hematoxylin.