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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 16601-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGCP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LEPRIHKIA ILGLGSSIGT PPEGITAEVL VVTSFDELQR RASEARGKIV VYNQPYINYS RTVQYRTQGA VEAAKVGALA SLIRSVASFS IYSPHTGIQE YQDGVPKIPT ACITVEDAEM MSRMASHGIK IVIQLKMGAK TYPDTDSFNT VAEITGSKYP EQVVLVSGHL DSWDVGQGAM DDGGGAFISW EALSLIKDLG LRPKRTLRLV LWTAEEQGGV GAFQYYQLHK VNISNYSLVM ESDAGTFLPT GLQFTGSEKA RAIMEEVMSL LQPLNITQVL SHGEGTDINF WIQAGVPGAS LLDDLYKYFF FHHSHGDTMT VMDPKQMNVA AAVWAVVSYV VADMEEMLPR S (123-472 aa encoded by BC020689)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.24 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human serum tissue were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of L02 cells using 16601-1-AP (PGCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PGCP (IP:16601-1-AP, 4ug; Detection:16601-1-AP 1:500) with mouse kidney tissue lysate 4000ug.
