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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB6459 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6459, RRID:AB_1580787
- Product name
- SMARCE1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of SMARCE1.
- Antigen sequence
C-PPTDPIPEDEKKE- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes.
Wang W, Chi T, Xue Y, Zhou S, Kuo A, Crabtree GR
Proceedings of the National Academy of Sciences of the United States of America 1998 Jan 20;95(2):492-8
Proceedings of the National Academy of Sciences of the United States of America 1998 Jan 20;95(2):492-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SMARCE1 polyclonal antibody (Cat # PAB6459) staining (0.2 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing SMARCE1 and probed with SMARCE1 polyclonal antibody (Cat # PAB6459) (mock transfection in first lane) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- In paraffin embedded human prostate shows nuclear staining in secretory epithelial cells. SMARCE1 polyclonal antibody (Cat # PAB6459) recommended concentration at 3-5 ug/mL.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
