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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP14501PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP14501PU-N, RRID:AB_1770835
- Product name
- anti Pumilio 2 / PUM2
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Ser182 of Human PUM2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot analysis of PUM2 (arrow) using PUM2 Antibody (Cat#AP14501PU-N). 293 cell lysates (2 µg/lane) either non-transfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2)(Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 3. Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(Bottom Histogram) compared to a Negative Control cell (Top Histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
