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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Western blot [2]
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- Product number
- NB100-387 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-387, RRID:AB_2173869
- Product name
- Rabbit Polyclonal PUM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A coding-independent function of an alternative Ube3a transcript during neuronal development.
MiR-134-dependent regulation of Pumilio-2 is necessary for homeostatic synaptic depression.
Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation.
Recombinant Adeno-Associated Virus-Mediated microRNA Delivery into the Postnatal Mouse Brain Reveals a Role for miR-134 in Dendritogenesis in Vivo.
Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.
Valluy J, Bicker S, Aksoy-Aksel A, Lackinger M, Sumer S, Fiore R, Wüst T, Seffer D, Metge F, Dieterich C, Wöhr M, Schwarting R, Schratt G
Nature neuroscience 2015 May;18(5):666-73
Nature neuroscience 2015 May;18(5):666-73
MiR-134-dependent regulation of Pumilio-2 is necessary for homeostatic synaptic depression.
Fiore R, Rajman M, Schwale C, Bicker S, Antoniou A, Bruehl C, Draguhn A, Schratt G
The EMBO journal 2014 Oct 1;33(19):2231-46
The EMBO journal 2014 Oct 1;33(19):2231-46
Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation.
Friend K, Brook M, Bezirci FB, Sheets MD, Gray NK, Seli E
The Biochemical journal 2012 Jul 1;445(1):93-100
The Biochemical journal 2012 Jul 1;445(1):93-100
Recombinant Adeno-Associated Virus-Mediated microRNA Delivery into the Postnatal Mouse Brain Reveals a Role for miR-134 in Dendritogenesis in Vivo.
Christensen M, Larsen LA, Kauppinen S, Schratt G
Frontiers in neural circuits 2010;3:16
Frontiers in neural circuits 2010;3:16
Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.
Padmanabhan K, Richter JD
Genes & development 2006 Jan 15;20(2):199-209
Genes & development 2006 Jan 15;20(2):199-209
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PUM2 Antibody [NB100-387] - Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody NB100-387 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY licence.
