GTX25621

antibody from GeneTex

Targeting: THRA AR7, EAR-7.1/EAR-7.2, ERBA, ERBA1, NR1A1, THRA1, THRA2, THRA3

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Gel shift (Data presented on provider website)

Antibody Data

Product number

GTX25621

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25621, RRID:AB_380602

Product name

Thyroid Hormone Receptor alpha antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Chicken/Avian, Sheep, Simian, Xenopus, Zebrafish

Host

Rabbit

References

Submitted references

Sex Differences in Brain Thyroid Hormone Levels during Early Post-Hatching Development in Zebra Finch (Taeniopygia guttata).

Yamaguchi S, Hayase S, Aoki N, Takehara A, Ishigohoka J, Matsushima T, Wada K, Homma KJ
PloS one 2017;12(1):e0169643

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Thyroid hormone suppresses expression of stathmin and associated tumor growth in hepatocellular carcinoma.

Tseng YH, Huang YH, Lin TK, Wu SM, Chi HC, Tsai CY, Tsai MM, Lin YH, Chang WC, Chang YT, Chen WJ, Lin KH
Scientific reports 2016 Dec 9;6:38756

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry analysis of Thyroid Hormone Receptor alpha-1 showing staining in the cytoplasm and nucleus of paraffin-treated human colon carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Thyroid Hormone Receptor alpha-1 polyclonal antibody (GTX25621) diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry analysis of Thyroid Hormone Receptor alpha-1 showing staining in the cytoplasm and nucleus of paraffin-treated human thyroid tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Thyroid Hormone Receptor alpha-1 polyclonal antibody (GTX25621) diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry analysis of Thyroid Hormone Receptor alpha-1 showing staining in the cytoplasm and nucleus of paraffin-treated rat thyroid tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Thyroid Hormone Receptor alpha-1 polyclonal antibody (GTX25621) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.