GTX22743

antibody from GeneTex

Targeting: THRA AR7, EAR-7.1/EAR-7.2, ERBA, ERBA1, NR1A1, THRA1, THRA2, THRA3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Gel shift (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX22743

Provider

GeneTex

Proper citation

GeneTex Cat#GTX22743, RRID:AB_374338

Product name

Thyroid Hormone Receptor antibody [C3]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Chicken/Avian, Simian, Xenopus

Host

Mouse

Storage

-20¢X C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot of human thyroid hormone receptor.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Thyroid Hormone Receptor in HeLa Cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Thyroid Hormone Receptor antibody [C3] at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a proper secondary antibody. Thyroid Hormone Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry was performed on normal biopsies of deparaffinized human thyroid tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed at a dilution of 1:20 with or without Thyroid Hormone Receptor antibody [C3] overnight at 4¢XC in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.