600-401-A38

antibody from Rockland Immunochemicals, Inc.

Targeting: THRA AR7, EAR-7.1/EAR-7.2, ERBA, ERBA1, NR1A1, THRA1, THRA2, THRA3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-A38

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-A38, RRID:AB_2202769

Product name

Anti-Thyroid Hormone Receptor alpha (THRA) (RABBIT) Antibody - 600-401-A38

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation.

Gao X, Lee HY, Li W, Platt RJ, Barrasa MI, Ma Q, Elmes RR, Rosenfeld MG, Lodish HF
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 19;114(38):10107-10112

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Impaired hair growth and wound healing in mice lacking thyroid hormone receptors.

Contreras-Jurado C, García-Serrano L, Martínez-Fernández M, Ruiz-Llorente L, Paramio JM, Aranda A
PloS one 2014;9(9):e108137

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Adipogenesis is differentially impaired by thyroid hormone receptor mutant isoforms.

Mishra A, Zhu XG, Ge K, Cheng SY
Journal of molecular endocrinology 2010 Apr;44(4):247-55

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA. Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.

Validation comment

Western Blot