210-401-B54
antibody from Rockland Immunochemicals, Inc.
Targeting: IL27
IL-27, IL-27A, IL27A, IL27p28, IL30, MGC71873, p28
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 210-401-B54 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#210-401-B54, RRID:AB_1961728
- Product name
- Anti-Mouse IL-27/p28 (RABBIT) Antibody - 210-401-B54
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Detection of recombinant IL27/p28 protein by Rockland's anti-Mouse IL-27/p28 antibody. Recombinant mouse IL27/p28 was loaded on to an SDS-PAGE gel at 0.25 µg and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
- Validation comment
- Western Blot