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- Antibody Data
- Antigen structure
- References [10]
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- Blocking/Neutralizing [1]
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- Product number
- AF2526 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-27 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-27 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse IL-27, recombinant human (rh) IL-12, and rhIL-12 p40 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q14213 (EBI-3 subunit), AAM34498 (p
28 subunit)- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Type I IFN augments IL-27-dependent TRIM25 expression to inhibit HBV replication.
Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation.
HIV and HIV-Tat inhibit LPS-induced IL-27 production in human macrophages by distinct intracellular signaling pathways.
RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production.
Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4.
IL-27 and type 2 immunity in asthmatic patients: association with severity, CXCL9, and signal transducer and activator of transcription signaling.
Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production.
Independent and interdependent immunoregulatory effects of IL-27, IFN-β, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis.
B cells as a therapeutic target for IFN-β in relapsing-remitting multiple sclerosis.
Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Tan G, Xiao Q, Song H, Ma F, Xu F, Peng D, Li N, Wang X, Niu J, Gao P, Qin FX, Cheng G
Cellular & molecular immunology 2018 Mar;15(3):272-281
Cellular & molecular immunology 2018 Mar;15(3):272-281
Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation.
Gregersen I, Sandanger Ø, Askevold ET, Sagen EL, Yang K, Holm S, Pedersen TM, Skjelland M, Krohg-Sørensen K, Hansen TV, Dahl TB, Otterdal K, Espevik T, Aukrust P, Yndestad A, Halvorsen B
PloS one 2017;12(11):e0188387
PloS one 2017;12(11):e0188387
HIV and HIV-Tat inhibit LPS-induced IL-27 production in human macrophages by distinct intracellular signaling pathways.
Gajanayaka N, O'Hara S, Konarski Y, Fernandes J, Muthumani K, Kozlowski M, Angel JB, Kumar A
Journal of leukocyte biology 2017 Sep;102(3):925-939
Journal of leukocyte biology 2017 Sep;102(3):925-939
RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production.
Sprokholt JK, Kaptein TM, van Hamme JL, Overmars RJ, Gringhuis SI, Geijtenbeek TBH
PLoS pathogens 2017 Nov;13(11):e1006738
PLoS pathogens 2017 Nov;13(11):e1006738
Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4.
Garg A, Trout R, Spector SA
Scientific reports 2017 Mar 24;7:44485
Scientific reports 2017 Mar 24;7:44485
IL-27 and type 2 immunity in asthmatic patients: association with severity, CXCL9, and signal transducer and activator of transcription signaling.
Xie M, Mustovich AT, Jiang Y, Trudeau JB, Ray A, Ray P, Hu H, Holguin F, Freeman B, Wenzel SE
The Journal of allergy and clinical immunology 2015 Feb;135(2):386-94
The Journal of allergy and clinical immunology 2015 Feb;135(2):386-94
Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production.
Gringhuis SI, Kaptein TM, Wevers BA, van der Vlist M, Klaver EJ, van Die I, Vriend LE, de Jong MA, Geijtenbeek TB
Nature communications 2014 Oct 3;5:5074
Nature communications 2014 Oct 3;5:5074
Independent and interdependent immunoregulatory effects of IL-27, IFN-β, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis.
Fitzgerald DC, Fonseca-Kelly Z, Cullimore ML, Safabakhsh P, Saris CJ, Zhang GX, Rostami A
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3225-34
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3225-34
B cells as a therapeutic target for IFN-β in relapsing-remitting multiple sclerosis.
Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4518-26
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4518-26
Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Kalliolias GD, Gordon RA, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):7047-56
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):7047-56
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-27 by Western Blot. Western blot shows lysates of MT-2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for the IL-27 EBI3/IL-27B at approximately 32 kDa and IL-27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-27 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IL-27 Antibody. Recombinant Human IL-27 (Catalog # 2526-IL) reduces the Encephalo-myocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL-27 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526). The ND50 is typically 1-5 µg/mL.
