BAF2526
antibody from R&D Systems
Targeting: IL27
IL-27, IL-27A, IL27A, IL27p28, IL30, MGC71873, p28
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BAF2526 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-27 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-27 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse IL-27, recombinant human (rh) IL-12, and rhIL-12 p40 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
Q14213 (EBI-3 subunit), AAM34498 (p
28 subunit)- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A soluble form of IL-27Rα is a natural IL-27 antagonist.
Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Dietrich C, Candon S, Ruemmele FM, Devergne O
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5382-9
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5382-9
Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Kalliolias GD, Gordon RA, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):7047-56
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):7047-56
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-27 by Western Blot. Western blot shows lysates of MT-2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-27 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2526) followed by Streptavidin-HRP (Catalog # DY998). Specific bands were detected for the IL-27 EBI3/IL-27B subunit at approximately 32 kDa and IL-27p28/IL27-A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.