Explore
Validate
LearnRQ5854
antibody from NSJ Bioreagents
Targeting: TRIM33
FLJ11429, KIAA1113, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- RQ5854 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TIF1 gamma Antibody / TRIM33
- Antibody type
- Monoclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Storage
- After reconstitution, the TRIM33 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) MCF7, 2) SW620 and 3) K562 lysate with TRIM33 antibody. Predicted molecular weight ~122 kDa but routinely observed at ~155 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with TRIM33 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer with TRIM33 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer with TRIM33 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HL-60 cells with TRIM33 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRIM33 antibody.
