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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- H00079977-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00079977-B01P, RRID:AB_1573891
- Product name
- GRHL2 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human GRHL2 protein.
- Antigen sequence
MSQESDNNKRLVALVPMPSDPPFNTRRAYTSEDEA
WKSYLENPLTAATKAMMSINGDEDSAAALGLLYDY
YKVPRDKRLLSVSKASDSQEDQEKRNCLGTSEAQS
NLSGGENRVQVLKTVPVNLSLNQDHLENSKREQYS
ISFPESSAIIPVSGITVVKAEDFTPVFMAPPVHYP
RGDGEEQRVVIFEQTQYDVPSLATHSAYLKDDQRS
TPDSTYSESFKDAATEKFRSASVGAEEYMYDQTSS
GTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITL
SETGDNKCFRHPISKVRSVVMVVFSEDKNRDEQLK
YWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEI
AYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVK
GLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKG
AERKIRDEERKQNRKKGKGQASQTQCNSSSDGKLA
AIPLQKKSDITYFKTMPDLHSQPVLFIPDVHFANL
QRTGQVYYNTDDEREGGSVLVKRMFRPMEEEFGPV
PSKQMKEEGTKRVLLYVRKETDDVFDALMLKSPTV
KGLMEAISEKYGLPVEKIAKLYKKSKKGILVNMDD
NIIEHYSNEDTFILNMESMVEGFKVTLMEI- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The p63 Gene Is Regulated by Grainyhead-like 2 (GRHL2) through Reciprocal Feedback and Determines the Epithelial Phenotype in Human Keratinocytes.
A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis.
Mehrazarin S, Chen W, Oh JE, Liu ZX, Kang KL, Yi JK, Kim RH, Shin KH, Park NH, Kang MK
The Journal of biological chemistry 2015 Aug 7;290(32):19999-20008
The Journal of biological chemistry 2015 Aug 7;290(32):19999-20008
A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis.
Dompe N, Rivers CS, Li L, Cordes S, Schwickart M, Punnoose EA, Amler L, Seshagiri S, Tang J, Modrusan Z, Davis DP
Proceedings of the National Academy of Sciences of the United States of America 2011 Oct 25;108(43):E943-51
Proceedings of the National Academy of Sciences of the United States of America 2011 Oct 25;108(43):E943-51
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GRHL2 expression in transfected 293T cell line (H00079977-T01) by GRHL2 MaxPab polyclonal antibody.Lane 1: GRHL2 transfected lysate(68.75 KDa).Lane 2: Non-transfected lysate.
- Validation comment
- Western Blot (Transfected lysate)
- Protocol
- Protocol
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GRHL2 MaxPab polyclonal antibody. Western Blot analysis of GRHL2 expression in A-431.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GRHL2 expression in transfected 293T cell line (H00079977-T01) by GRHL2 MaxPab polyclonal antibody.Lane 1: GRHL2 transfected lysate(68.75 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to GRHL2 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
