Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [2]
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Validation data
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- Product number
- RQ6869 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- FUS Antibody / TLS
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the TLS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human K562, 3) human 293T, 4) human Caco-2, 5) human Jurkat, 6) human HL60, 7) human HepG2, 8) human SH-SY5Y, 9) rat brain, 10) rat PC-12 and 11) mouse NIH 3T3 cell lysate with TLS antibody. Predicted molecular weight ~53 kDa but routinely observed at up to ~75 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with TLS antibody (green) and Phalloidin (red). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer tissue with TLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse colon tissue with TLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat colon tissue with TLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with TLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TLS antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse ANA-1 cells with TLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TLS antibody.