MA5-34902

antibody from Invitrogen Antibodies

Targeting: NFIB NFI-RED, NFIB2, NFIB3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34902

Provider

Invitrogen Antibodies

Product name

NFIB Monoclonal Antibody (1H2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: SH-SY-5Y, LO-2, rat heart tissue, human colon cancer tissue, human prostate tissue, human pancreas tissue, mouse liver tissue.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1H2

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFIB NF1B2 in SH-SY5Y cell lysates. Samples were incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NFIB NF1B2 in LO-2 cells. Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NFIB NF1B2 in paraffin-embedded mouse liver tissue. Samples were incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NFIB NF1B2 in paraffin-embedded rat heart tissue. Samples were incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NFIB NF1B2 in paraffin-embedded mouse liver tissue. Samples were incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NFIB NF1B2 in paraffin-embedded human prostate tissue. Samples were incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NFIB NF1B2 in paraffin-embedded human pancreas tissue. Samples were incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NFIB NF1B2 in paraffin-embedded human colon cancer tissue. Samples were incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of NFIB NF1B2 in SH-SY-5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG.