HPA003956

antibody from Atlas Antibodies

Targeting: NFIB NFI-RED, NFIB2, NFIB3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA003956

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003956, RRID:AB_1854424

Product name

Anti-NFIB

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human NFIB, Gene description: nuclear factor I/B, Alternative Gene Names: NFI-RED, NFIB2, NFIB3, Validated applications: ICC, WB, Uniprot ID: O00712, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

PAX6 does not regulate Nfia and Nfib expression during neocortical development

Bunt J, Lim J, Zhao L, Mason S, Richards L
Scientific Reports 2015 May;5

---

NFI transcription factors interact with FOXA1 to regulate prostate-specific gene expression.

Grabowska MM, Elliott AD, DeGraff DJ, Anderson PD, Anumanthan G, Yamashita H, Sun Q, Friedman DB, Hachey DL, Yu X, Sheehan JH, Ahn JM, Raj GV, Piston DW, Gronostajski RM, Matusik RJ
Molecular endocrinology (Baltimore, Md.) 2014 Jun;28(6):949-64

---

MiR-153 targets the nuclear factor-1 family and protects against teratogenic effects of ethanol exposure in fetal neural stem cells.

Tsai PC, Bake S, Balaraman S, Rawlings J, Holgate RR, Dubois D, Miranda RC
Biology open 2014 Jul 25;3(8):741-58

---

NFIB-mediated repression of the epigenetic factor Ezh2 regulates cortical development.

Piper M, Barry G, Harvey TJ, McLeay R, Smith AG, Harris L, Mason S, Stringer BW, Day BW, Wray NR, Gronostajski RM, Bailey TL, Boyd AW, Richards LJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 19;34(8):2921-30

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line SH-SY5Y.

Sample type

Human

Protocol

Protocol

Appendix

protocol-icc-if-protocol.pdf?language=en&utm_campaign=antibodypedia

Show more

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.

Sample type

Human

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong nuclear positivity in myocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neurons.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows moderate nuclear positivity in squamous epithelial cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.

Sample type

HUMAN