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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324363 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal SREBF2 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal SREBF2 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SREBF2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SREBF2 Antibody (Center) (Cat. #TA324363) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SREBF2 antibody detected the SREBF2 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cell stained with SREBF2 Antibody (Center)(Cat#TA324363).U251 cells were incubated with SREBF2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).SREBF2 immunoreactivity is localized to Nucleus and Nucleolus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SREBF2 antibody (Center) (Cat. #TA324363) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SREBF2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
