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- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Western blot [1]
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- Product number
- NBP1-54446 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal SREBP2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Recognizes the precursor and mature forms of human SREBP2.
- Reactivity
- Human, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The AAA ATPase VPS4/SKD1 regulates endosomal cholesterol trafficking independently of ESCRT-III.
An essential role of Hrs/Vps27 in endosomal cholesterol trafficking.
Akt acutely activates the cholesterogenic transcription factor SREBP-2.
Insig-dependent ubiquitination and degradation of 3-hydroxy-3-methylglutaryl coenzyme a reductase stimulated by delta- and gamma-tocotrienols.
Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterol.
Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro.
Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens.
Cholesterol and 25-hydroxycholesterol inhibit activation of SREBPs by different mechanisms, both involving SCAP and Insigs.
Platelet-derived growth factor stimulates membrane lipid synthesis through activation of phosphatidylinositol 3-kinase and sterol regulatory element-binding proteins.
Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain.
Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins.
The hypocholesterolemic agent LY295427 up-regulates INSIG-1, identifying the INSIG-1 protein as a mediator of cholesterol homeostasis through SREBP.
Sterol regulatory element-binding protein-1 interacts with the nuclear thyroid hormone receptor to enhance acetyl-CoA carboxylase-alpha transcription in hepatocytes.
The hypocholesterolemic agent LY295427 reverses suppression of sterol regulatory element-binding protein processing mediated by oxysterols.
Du X, Kazim AS, Dawes IW, Brown AJ, Yang H
Traffic (Copenhagen, Denmark) 2013 Jan;14(1):107-19
Traffic (Copenhagen, Denmark) 2013 Jan;14(1):107-19
An essential role of Hrs/Vps27 in endosomal cholesterol trafficking.
Du X, Kazim AS, Brown AJ, Yang H
Cell reports 2012 Jan 26;1(1):29-35
Cell reports 2012 Jan 26;1(1):29-35
Akt acutely activates the cholesterogenic transcription factor SREBP-2.
Luu W, Sharpe LJ, Stevenson J, Brown AJ
Biochimica et biophysica acta 2012 Feb;1823(2):458-64
Biochimica et biophysica acta 2012 Feb;1823(2):458-64
Insig-dependent ubiquitination and degradation of 3-hydroxy-3-methylglutaryl coenzyme a reductase stimulated by delta- and gamma-tocotrienols.
Song BL, DeBose-Boyd RA
The Journal of biological chemistry 2006 Sep 1;281(35):25054-61
The Journal of biological chemistry 2006 Sep 1;281(35):25054-61
Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterol.
Song BL, Javitt NB, DeBose-Boyd RA
Cell metabolism 2005 Mar;1(3):179-89
Cell metabolism 2005 Mar;1(3):179-89
Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro.
Sun LP, Li L, Goldstein JL, Brown MS
The Journal of biological chemistry 2005 Jul 15;280(28):26483-90
The Journal of biological chemistry 2005 Jul 15;280(28):26483-90
Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens.
Zhang Y, Hillgartner FB
The Journal of nutrition 2004 Sep;134(9):2205-10
The Journal of nutrition 2004 Sep;134(9):2205-10
Cholesterol and 25-hydroxycholesterol inhibit activation of SREBPs by different mechanisms, both involving SCAP and Insigs.
Adams CM, Reitz J, De Brabander JK, Feramisco JD, Li L, Brown MS, Goldstein JL
The Journal of biological chemistry 2004 Dec 10;279(50):52772-80
The Journal of biological chemistry 2004 Dec 10;279(50):52772-80
Platelet-derived growth factor stimulates membrane lipid synthesis through activation of phosphatidylinositol 3-kinase and sterol regulatory element-binding proteins.
Demoulin JB, Ericsson J, Kallin A, Rorsman C, Rönnstrand L, Heldin CH
The Journal of biological chemistry 2004 Aug 20;279(34):35392-402
The Journal of biological chemistry 2004 Aug 20;279(34):35392-402
Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain.
Sever N, Yang T, Brown MS, Goldstein JL, DeBose-Boyd RA
Molecular cell 2003 Jan;11(1):25-33
Molecular cell 2003 Jan;11(1):25-33
Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins.
Yabe D, Brown MS, Goldstein JL
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12753-8
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12753-8
The hypocholesterolemic agent LY295427 up-regulates INSIG-1, identifying the INSIG-1 protein as a mediator of cholesterol homeostasis through SREBP.
Janowski BA
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12675-80
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12675-80
Sterol regulatory element-binding protein-1 interacts with the nuclear thyroid hormone receptor to enhance acetyl-CoA carboxylase-alpha transcription in hepatocytes.
Yin L, Zhang Y, Hillgartner FB
The Journal of biological chemistry 2002 May 31;277(22):19554-65
The Journal of biological chemistry 2002 May 31;277(22):19554-65
The hypocholesterolemic agent LY295427 reverses suppression of sterol regulatory element-binding protein processing mediated by oxysterols.
Janowski BA, Shan B, Russell DW
The Journal of biological chemistry 2001 Nov 30;276(48):45408-16
The Journal of biological chemistry 2001 Nov 30;276(48):45408-16
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SREBP2 Antibody (1D2) [NBP1-54446] - Lane1: HEp-2 cells. Lane 2: MCF-7 cells. Lane 3: LNCaP cells. Lane 4: HT1080 cells. Lane 5: ZR75-1 cells.
