GTX82865

antibody from GeneTex

Targeting: SREBF2 bHLHd2, SREBP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX82865

Provider

GeneTex

Proper citation

GeneTex Cat#GTX82865, RRID:AB_626055

Product name

SREBP2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis.

Kang J, Lienhard M, Pastor WA, Chawla A, Novotny M, Tsagaratou A, Lasken RS, Thompson EC, Surani MA, Koralov SB, Kalantry S, Chavez L, Rao A
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 4;112(31):E4236-45

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of SREBP2 in 25 ug of mouse liver, rat liver and HepG2 lysates. Proteins were transferred to a PVDF membrane and blocked at 4¢XC overnight. The membrane was probed with SREBP2 antibody at a dilution of 1:500 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of SREBP2 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed without or with SREBP2 antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4¢XC in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.