GTX111045

antibody from GeneTex

Targeting: HSPA1L HSP70-HOM, hum70t

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX111045

Provider

GeneTex

Proper citation

GeneTex Cat#GTX111045, RRID:AB_1950521

Product name

HSP70 1L antibody [N1N3]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Whole exome sequencing reveals HSPA1L as a genetic risk factor for spontaneous preterm birth.

Huusko JM, Karjalainen MK, Graham BE, Zhang G, Farrow EG, Miller NA, Jacobsson B, Eidem HR, Murray JC, Bedell B, Breheny P, Brown NW, Bødker FL, Litterman NK, Jiang PP, Russell L, Hinds DA, Hu Y, 23andMe Research Team, Rokas A, Teramo K, Christensen K, Williams SM, Rämet M, Kingsmore SF, Ryckman KK, Hallman M, Muglia LJ
PLoS genetics 2018 Jul;14(7):e1007394

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Regulation of CLC-1 chloride channel biosynthesis by FKBP8 and Hsp90β.

Peng YJ, Huang JJ, Wu HH, Hsieh HY, Wu CY, Chen SC, Chen TY, Tang CY
Scientific reports 2016 Sep 1;6:32444

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TC-N19, a novel dual inhibitor of EGFR and cMET, efficiently overcomes EGFR-TKI resistance in non-small-cell lung cancer cells.

Wu DW, Chen TC, Huang HS, Lee H
Cell death & disease 2016 Jun 30;7(6):e2290

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Shikonin-enhanced cell immunogenicity of tumor vaccine is mediated by the differential effects of DAMP components.

Lin TJ, Lin HT, Chang WT, Mitapalli S P, Hsiao PW, Yin SY, Yang NS
Molecular cancer 2015 Sep 24;14:174

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX111045 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Rat tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A549, using HSP70 1L(GTX111045) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HSP70 1L antibody [N1N3] detects HSP70 1L protein at cytoplasm by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HSP70 1L protein stained by HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:400.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using HSP70 1L(GTX111045) antibody at 1:500 dilution.