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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
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- Product number
- NB500-178 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-178, RRID:AB_10002835
- Product name
- Rabbit Polyclonal Numb Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This is specific for all four isoforms of the NUMB protein.
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 3.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Inadequate DNA Damage Repair Promotes Mammary Transdifferentiation, Leading to BRCA1 Breast Cancer.
Proteomic analysis reveals novel ligands and substrates for LNX1 E3 ubiquitin ligase.
Decreased Anxiety-Related Behaviour but Apparently Unperturbed NUMB Function in Ligand of NUMB Protein-X (LNX) 1/2 Double Knockout Mice.
Wang H, Xiang D, Liu B, He A, Randle HJ, Zhang KX, Dongre A, Sachs N, Clark AP, Tao L, Chen Q, Botchkarev VV Jr, Xie Y, Dai N, Clevers H, Li Z, Livingston DM
Cell 2019 Jun 27;178(1):135-151.e19
Cell 2019 Jun 27;178(1):135-151.e19
Proteomic analysis reveals novel ligands and substrates for LNX1 E3 ubiquitin ligase.
Lenihan JA, Saha O, Young PW
PloS one 2017;12(11):e0187352
PloS one 2017;12(11):e0187352
Decreased Anxiety-Related Behaviour but Apparently Unperturbed NUMB Function in Ligand of NUMB Protein-X (LNX) 1/2 Double Knockout Mice.
Lenihan JA, Saha O, Heimer-McGinn V, Cryan JF, Feng G, Young PW
Molecular neurobiology 2017 Dec;54(10):8090-8109
Molecular neurobiology 2017 Dec;54(10):8090-8109
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
