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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501614 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501614, RRID:AB_11124181
- Product name
- NUMB mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)
- Antibody type
- Monoclonal
- Description
- NUMB mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NUMB
- Isotype
- IgG
- Antibody clone number
- OTI1A7
- Vial size
- 100 µl
- Concentration
- 0.6 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-NUMB monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-NUMB mouse monoclonal antibody (TA501614) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUMB(RC216230 ).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC216230 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUMB antibody(TA501614), and then analyzed by flow cytometry.
- Validation comment
- FC
