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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 60137-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUMB Monoclonal Antibody (2G12B7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71, and P72, but can not recognize NUMBL.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G12B7
- Vial size
- 150 µL
- Concentration
- 2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 60137-1-IG (NUMB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells using 60137-1-IG (NUMB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat brain tissue slide using 60137-1-IG ( NUMB Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat brain tissue slide using 60137-1-IG ( NUMB Antibody) at dilution of 1:200 (under 40x lens).
