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LearnNBP2-16542
antibody from Novus Biologicals
Targeting: FUBP1
FBP, FUBP
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- NBP2-16542 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal FUBP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FUBP1 Antibody [NBP2-16542] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FUBP1 Antibody [NBP2-16542] - Hela whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FUBP1 Antibody [NBP2-16542] - 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 ug of FUBP-1 antibody 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FUBP1 Antibody [NBP2-16542] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - : Paraffin-embedded mouse hind brain. FUBP1 antibody dilution: 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - Paraffin-embedded mouse testis. FUBP1 antibody dilution: 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: FUBP1 Antibody [NBP2-16542] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.
