- PA5-28061 - Invitrogen Antibodies FUBP1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of FUBP1 using 30 µg of A) Jurkat (B) K562 (C) THP-1 and D) NCI-H929 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a FUBP1 polyclonal antibody (Product # PA5-28061) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot analysis of FUBP1 was performed by separating 30 µg of Hela whole cell and nuclear extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a FUBP1 Polyclonal Antibody (Product # PA5-28061) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot analysis of FUBP1 was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a FUBP1 Polyclonal Antibody (Product # PA5-28061) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot using FUBP1 Polyclonal Antibody (Product # PA5-28061). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 Polyclonal Antibody (Product # PA5-28061) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FUBP1 Polyclonal Antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 Polyclonal Antibody (Product # PA5-28061) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FUBP1 Polyclonal Antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. FUBP1 Polyclonal Antibody (Product # PA5-28061) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FUBP1 Polyclonal Antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. FUBP1 Polyclonal Antibody (Product # PA5-28061) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or FUBP1 Polyclonal Antibody (Product # PA5-28061). The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: RNA immunoprecipitation (RIP) western of FUBP1 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal FUBP1 antibody (Product # PA5-28061) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FUBP-1 antibody immunoprecipitates FUBP-1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 µg of FUBP-1 antibody (Product # PA5-28061) 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody (Product # PA5-28061) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.

PA5-28061