Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP2-36751 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TUFM Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human TUFM antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Rat
- Host
- Mouse
- Antigen sequence
RDLEKPFLLPVEAVY
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references PPEF2 Opposes PINK1-Mediated Mitochondrial Quality Control by Dephosphorylating Ubiquitin.
Wall CE, Rose CM, Adrian M, Zeng YJ, Kirkpatrick DS, Bingol B
Cell reports 2019 Dec 3;29(10):3280-3292.e7
Cell reports 2019 Dec 3;29(10):3280-3292.e7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TUFM Antibody (CL2242) [NBP2-36751] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TUFM Antibody (CL2242) [NBP2-36751] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TUFM Antibody (CL2242) [NBP2-36751] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TUFM antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751] - Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate staining in glomerular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.