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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- A05537 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ZIC1/2/3 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for ZIC1 detection. Host: Rabbit.Size: 100μl. Tested applications: IHC. Reactive species: Human. ZIC1 information: Molecular Weight: 48309 MW; Subcellular Localization: Nucleus. Cytoplasm . Localizes in the cytoplasm in presence of MDFIC overexpression; Tissue Specificity: CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 100μl
- Concentration
- 1.0 mg/mL by UV absorbance at 280 nm
- Storage
- Store at -20°C for one year, at 4°C for one month. We suggest the antibody be aliquoted into small vials and stored frozen at -20°C upon receiving. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Western blot analysis of Zic1 expression in mouse cerebellum extracts at various time points. Zic1 was detected using rabbit anti- Zic1 Ion exchange chromatography purified polyclonal antibody (Catalog #A05537) at 1:5000. HRP conjugated anti-Rabbit IgG (Chemicon) was used at a 1,000 dilution. Personnel communication, K.H. Herzog.
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