PAB28035

antibody from Abnova Corporation

Targeting: ECSIT SITPEC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28035

Provider

Abnova Corporation

Product name

ECSIT polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ECSIT

Antigen sequence

HMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGI
QSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYY
HILRADLLPPEEREVEETPEEWNLYYPM


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4 Lane 2: U-251 MG Lane 3: Human Plasma Lane 4: Liver Lane 5: Tonsil with ECSIT polyclonal antibody ( Cat # PAB28035 ) at 1:100 - 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with ECSIT polyclonal antibody ( Cat # PAB28035 ) at 1-4 ug/ml dilution shows positivity in nucleus but not nucleoli & cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with ECSIT polyclonal antibody ( Cat # PAB28035 ) shows strong cytoplasmic positivity in glandular cells at 1:200 - 1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)