PAB10005

antibody from Abnova Corporation

Targeting: ECSIT SITPEC

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10005

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10005, RRID:AB_1674720

Product name

ECSIT polyclonal antibody

Antibody type

Polyclonal

Antigen

A synthetic peptide corresponding to C-terminus of human ECSIT.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

100 μg

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Toll-like receptors.

Takeda K, Kaisho T, Akira S
Annual review of immunology 2003;21:335-76

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Functional diversity and regulation of different interleukin-1 receptor-associated kinase (IRAK) family members.

Janssens S, Beyaert R
Molecular cell 2003 Feb;11(2):293-302

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TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression.

Vogel SN, Fitzgerald KA, Fenton MJ
Molecular interventions 2003 Dec;3(8):466-77

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using ECSIT polyclonal antibody (Cat # PAB10005) shows detection of ECSIT in human heart tissue lysate.The membrane was probed with the primary antibody diluted to 1 : 2000 (lane 1), 1 : 1000 (lane 2), and 1 : 500 (lane 3) respectively.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry using ECSIT polyclonal antibody (Cat # PAB10005) shows staining of ECSIT in mouse heart tissue. Paraffin embedded formalin fixed tissue was reacted with primary antibody diluted 1 : 500.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)