PA5-60183

antibody from Invitrogen Antibodies

Targeting: ECSIT SITPEC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-60183

Provider

Invitrogen Antibodies

Product name

ECSIT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: HMEPDLSARV TIYQVPLPKD STGAADPPQP HIVGIQSPDQ QAALARHNPA RPVFVEGPFS LWLRNKCVYY HILRADLLPP EEREVEETPE EWNLYYPM

Concentration

0.3 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ECSIT in human cell lines PC-3 and HeLa using a ECSIT Polyclonal Antibody (Product # PA5-60183). Corresponding ECSIT RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ECSIT in mouse cell line NIH-3T3 and rat cell line NBT-II using a ECSIT Polyclonal Antibody (Product # PA5-60183).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ECSIT in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using an ECSIT Polyclonal Antibody (Product # PA5-60183).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ECSIT in human colon tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using an ECSIT Polyclonal Antibody (Product # PA5-60183).