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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6106 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6106, RRID:AB_1574659
- Product name
- ELMO2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of ELMO2.
- Antigen sequence
C-PKEPSSYDFVYHYG- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration.
Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, Walk SF, Nemergut ME, Macara IG, Francis R, Schedl T, Qin Y, Van Aelst L, Hengartner MO, Ravichandran KS
Cell 2001 Oct 5;107(1):27-41
Cell 2001 Oct 5;107(1):27-41
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELMO2 polyclonal antibody (Cat # PAB6106) staining (2 ug/mL) of human brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELMO2 polyclonal antibody (Cat # PAB6106)(3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
