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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 25086-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIPA1L1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SQLAPSFSS SSSSSSGPRS FYPRQGATSK YLIGWKKPEG TINSVGFMDT RKRHQSDGNE IAHTRLRAST RDLRASPKPT SKSTIEEDLK KLIDLESPTP ESQKSFKFHA LSSPQSPFPS TPTSRRALHR TLSDESIYNS QREHFFTSRA SLLDQALPND VLFSSTYPSL PKSLPLRRPS YTLGMKSLHG EFSASDSSLT DIQETRRQPM PDPGLMPLPD TAADLDWSNL VDAAKAYEVQ RASFFAASDE NHRPLSAASN SDQLEDQALP QMKPYSSKDS SPTLASKVDQ LEGMLKMLRE DLKKEKEDKA HLQAEVQHLR EDNLRLQEES QNASDKLKKF TEWVFNTIDM S (1454-1803 aa encoded by BC146777)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 25086-1-AP ( SIPA1L1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 25086-1-AP ( SIPA1L1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 25086-1-AP ( SIPA1L1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SIPA1L1 (IP:25086-1-AP, 4ug; Detection:25086-1-AP 1:1500) with mouse brain tissue lysate 4000ug.
