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LearnMAB225-100
antibody from R&D Systems
Targeting: TNFRSF1A
CD120a, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR60
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB225-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF RI/TNFRSF1A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human (rh) TNF RII, rmTNF RI, or rmTNF RII.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_001056- Isotype
- IgG
- Antibody clone number
- 16803
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human TNF-Luc reporter mouse: A new model to quantify inflammatory responses.
Low doses of LPS exacerbate the inflammatory response and trigger death on TLR3-primed human monocytes.
ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage.
ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor.
TNFα promotes CAR-dependent migration of leukocytes across epithelial monolayers.
High efficiency cell-specific targeting of cytokine activity.
Apoptotic signaling through Fas and TNF receptors ameliorates GVHD in mobilized peripheral blood grafts.
Enhancement of TWIK-related acid-sensitive potassium channel 3 (TASK3) two-pore domain potassium channel activity by tumor necrosis factor α.
Tumor necrosis factor stimulates osteoclastogenesis from human bone marrow cells under hypoxic conditions.
The transmembrane domains of TNF-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2 co-regulate apoptotic signaling capacity.
IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Death receptor expression is associated with poor response to chemotherapy and shorter survival in metastatic ovarian carcinoma.
Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals.
A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
A role for tumor necrosis factor alpha in death of dopaminergic neurons following neural transplantation.
Minshawi F, White MRH, Muller W, Humphreys N, Jackson D, Campbell BJ, Adamson A, Papoutsopoulou S
Scientific reports 2019 Jan 17;9(1):193
Scientific reports 2019 Jan 17;9(1):193
Low doses of LPS exacerbate the inflammatory response and trigger death on TLR3-primed human monocytes.
Monguió-Tortajada M, Franquesa M, Sarrias MR, Borràs FE
Cell death & disease 2018 May 1;9(5):499
Cell death & disease 2018 May 1;9(5):499
ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage.
Chakraborty A, Dorsett KA, Trummell HQ, Yang ES, Oliver PG, Bonner JA, Buchsbaum DJ, Bellis SL
The Journal of biological chemistry 2018 Jan 19;293(3):984-994
The Journal of biological chemistry 2018 Jan 19;293(3):984-994
ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor.
Holdbrooks AT, Britain CM, Bellis SL
The Journal of biological chemistry 2018 Feb 2;293(5):1610-1622
The Journal of biological chemistry 2018 Feb 2;293(5):1610-1622
TNFα promotes CAR-dependent migration of leukocytes across epithelial monolayers.
Morton PE, Hicks A, Ortiz-Zapater E, Raghavan S, Pike R, Noble A, Woodfin A, Jenkins G, Rayner E, Santis G, Parsons M
Scientific reports 2016 May 19;6:26321
Scientific reports 2016 May 19;6:26321
High efficiency cell-specific targeting of cytokine activity.
Garcin G, Paul F, Staufenbiel M, Bordat Y, Van der Heyden J, Wilmes S, Cartron G, Apparailly F, De Koker S, Piehler J, Tavernier J, Uzé G
Nature communications 2014;5:3016
Nature communications 2014;5:3016
Apoptotic signaling through Fas and TNF receptors ameliorates GVHD in mobilized peripheral blood grafts.
Mizrahi K, Yaniv I, Ash S, Stein J, Askenasy N
Bone marrow transplantation 2014 May;49(5):640-8
Bone marrow transplantation 2014 May;49(5):640-8
Enhancement of TWIK-related acid-sensitive potassium channel 3 (TASK3) two-pore domain potassium channel activity by tumor necrosis factor α.
El Hachmane MF, Rees KA, Veale EL, Sumbayev VV, Mathie A
The Journal of biological chemistry 2014 Jan 17;289(3):1388-401
The Journal of biological chemistry 2014 Jan 17;289(3):1388-401
Tumor necrosis factor stimulates osteoclastogenesis from human bone marrow cells under hypoxic conditions.
Nomura T, Aoyama M, Waguri-Nagaya Y, Goto Y, Suzuki M, Miyazawa K, Asai K, Goto S
Experimental cell research 2014 Feb 15;321(2):167-77
Experimental cell research 2014 Feb 15;321(2):167-77
The transmembrane domains of TNF-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2 co-regulate apoptotic signaling capacity.
Neumann S, Bidon T, Branschädel M, Krippner-Heidenreich A, Scheurich P, Doszczak M
PloS one 2012;7(8):e42526
PloS one 2012;7(8):e42526
IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
Kolliputi N, Waxman AB
American journal of respiratory cell and molecular biology 2009 Mar;40(3):314-24
American journal of respiratory cell and molecular biology 2009 Mar;40(3):314-24
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
The Journal of allergy and clinical immunology 2009 Jan;123(1):239-48
The Journal of allergy and clinical immunology 2009 Jan;123(1):239-48
Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Renaud SJ, Sullivan R, Graham CH
Placenta 2009 Apr;30(4):313-9
Placenta 2009 Apr;30(4):313-9
Death receptor expression is associated with poor response to chemotherapy and shorter survival in metastatic ovarian carcinoma.
Dong HP, Kleinberg L, Silins I, Flørenes VA, Tropé CG, Risberg B, Nesland JM, Davidson B
Cancer 2008 Jan 1;112(1):84-93
Cancer 2008 Jan 1;112(1):84-93
Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals.
Seal S, Hockenbery DM, Spaulding EY, Kiem HP, Abbassi N, Deeg HJ
Experimental hematology 2008 Dec;36(12):1660-72
Experimental hematology 2008 Dec;36(12):1660-72
A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK
Cell death and differentiation 2006 Oct;13(10):1789-801
Cell death and differentiation 2006 Oct;13(10):1789-801
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Ebert EC, Mehta V
Cellular immunology 2006 May;241(1):7-13
Cellular immunology 2006 May;241(1):7-13
A role for tumor necrosis factor alpha in death of dopaminergic neurons following neural transplantation.
Clarke DJ, Branton RL
Experimental neurology 2002 Jul;176(1):154-62
Experimental neurology 2002 Jul;176(1):154-62
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF RI/TNFRSF1A Inhibition of TNF-alpha-induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF-alpha (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225). The ND50 is typically 0.008-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
