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antibody from R&D Systems
Targeting: TNFRSF1A
CD120a, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR60
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF-425-PB - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TNF RI/TNFRSF1A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human TNF RI is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P25118- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain.
Death-domain dimerization-mediated activation of RIPK1 controls necroptosis and RIPK1-dependent apoptosis.
Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation.
Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury.
Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
Transforming growth factor β-activated kinase 1 signaling pathway critically regulates myocardial survival and remodeling.
Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
IL-1beta augments TNF-alpha-mediated inflammatory responses from lung epithelial cells.
The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins.
Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity.
Polykratis A, Martens A, Eren RO, Shirasaki Y, Yamagishi M, Yamaguchi Y, Uemura S, Miura M, Holzmann B, Kollias G, Armaka M, van Loo G, Pasparakis M
Nature cell biology 2019 Jun;21(6):731-742
Nature cell biology 2019 Jun;21(6):731-742
Death-domain dimerization-mediated activation of RIPK1 controls necroptosis and RIPK1-dependent apoptosis.
Meng H, Liu Z, Li X, Wang H, Jin T, Wu G, Shan B, Christofferson DE, Qi C, Yu Q, Li Y, Yuan J
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 27;115(9):E2001-E2009
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 27;115(9):E2001-E2009
Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation.
Arokiasamy S, Zakian C, Dilliway J, Wang W, Nourshargh S, Voisin MB
Scientific reports 2017 Mar 13;7:44189
Scientific reports 2017 Mar 13;7:44189
Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury.
Martens S, Jeong M, Tonnus W, Feldmann F, Hofmans S, Goossens V, Takahashi N, Bräsen JH, Lee EW, Van der Veken P, Joossens J, Augustyns K, Fulda S, Linkermann A, Song J, Vandenabeele P
Cell death & disease 2017 Jun 29;8(6):e2904
Cell death & disease 2017 Jun 29;8(6):e2904
Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
Freeman BE, Meyer C, Slifka MK
Journal of virology 2014 Jul;88(13):7474-84
Journal of virology 2014 Jul;88(13):7474-84
Transforming growth factor β-activated kinase 1 signaling pathway critically regulates myocardial survival and remodeling.
Li L, Chen Y, Doan J, Murray J, Molkentin JD, Liu Q
Circulation 2014 Dec 9;130(24):2162-72
Circulation 2014 Dec 9;130(24):2162-72
Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):2024-33
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):2024-33
Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
The Journal of experimental medicine 2012 Jun 4;209(6):1219-34
The Journal of experimental medicine 2012 Jun 4;209(6):1219-34
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
The Journal of clinical investigation 2009 Oct;119(10):2954-64
The Journal of clinical investigation 2009 Oct;119(10):2954-64
IL-1beta augments TNF-alpha-mediated inflammatory responses from lung epithelial cells.
Saperstein S, Chen L, Oakes D, Pryhuber G, Finkelstein J
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2009 May;29(5):273-84
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2009 May;29(5):273-84
The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
Nature immunology 2008 Sep;9(9):1047-54
Nature immunology 2008 Sep;9(9):1047-54
P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins.
Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P
The Journal of biological chemistry 2006 Oct 13;281(41):30684-96
The Journal of biological chemistry 2006 Oct 13;281(41):30684-96
Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity.
LaBue M, Colburn KK, Green LM
Thyroid : official journal of the American Thyroid Association 2004 Apr;14(4):249-62
Thyroid : official journal of the American Thyroid Association 2004 Apr;14(4):249-62
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RI/TNFRSF1A at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TNF RI/TNFRSF1A in L-929 Mouse Cell Line by Flow Cytometry. L-929 mouse fibroblast cell line was stained with Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB, filled histo-gram) or control antibody (Catalog # AB-108-C, open histo-gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
