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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX111167 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111167, RRID:AB_1949577
- Product name
- alpha Actinin 2 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX111167 diluted at 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 7.5% SDS PAGE GTX111167 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX111167) diluted at 1:5000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2(GTX111167) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Actinin 2 antibody [N2C1], Internal detects alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:250.
