PAB20419

antibody from Abnova Corporation

Targeting: ACTN2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20419

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20419, RRID:AB_10964000

Product name

ACTN2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ACTN2.

Antigen sequence

AHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNI
RISSSNPYSTVTMDELRTKWDKVKQLVPIRDQSLQ
EELARQHANERLRRQFAAQANAIGPWIQNKMEEIA
RSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLE
GD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with ACTN2 polyclonal antibody (Cat # PAB20419) at 1:250-1:500 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with ACTN2 polyclonal antibody (Cat # PAB20419) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-II

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle with ACTN2 polyclonal antibody (Cat # PAB20419) shows strong cytoplasmic positivity in myocytes at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)