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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB9830-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat alpha Actinin 2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human ACTN2 in direct ELISAs.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P35609- Isotype
- IgG
- Antibody clone number
- 2285D
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat alpha Actinin 2 by Western Blot. Western blot shows lysates of human skeletal muscle tissue, mouse skeletal muscle tissue, mouse heart tissue, and rat skeletal muslce tissue. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for alpha Actinin 2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Alpha Actinin 2 in C2C12 mouse myoblast cell line. Alpha Actinin 2 was detected in immersion fixed C2C12 mouse myoblast cell line using Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
