14221-1-AP

antibody from Invitrogen Antibodies

Targeting: ACTN2

Immunoprecipitation

Antibody data

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Validation data

Product number: 14221-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: ACTN2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: MNQIEPGVQ YNYVYDEDEY MIQEEEWDRD LLLDPAWEKQ QRKTFTAWCN SHLRKAGTQI ENIEEDFRNG LKLMLLLEVI SGERLPKPDR GKMRFHKIAN VNKALDYIAS KGVKLVSIGA EEIVDGNVKM TLGMIWTIIL RFAIQDISVE ETSAKEGLLL WCQRKTAPYR NVNIQNFHTS WKDGLGLCAL IHRHRPDLID YSKLNKDDPI GNINLAMEIA EKHLDIPKML DAEDIVNTPK PDERAIMTYV SCFYHAFAGA EQAETAANRI CKVLAVNQEN ERLMEEYERL ASELLEWIRR TI (1-301 aa encoded by BC051770)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.19 mg/mL
Storage: -20°C

ACTN2 protein structure - 14221-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart tissue slide using 14221-1-AP (ACTN2 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart tissue slide using 14221-1-AP (ACTN2 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 14221-1-AP (ACTN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using (Product # 14221-1-AP) (ACTN2 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).