NBP1-32462

antibody from Novus Biologicals

Targeting: ACTN2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-32462

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-32462, RRID:AB_2221551

Product name

Rabbit Polyclonal Alpha Actinin 2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Porcine

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Acute AT(1)R blockade prevents isoproterenol-induced injury in mdx hearts.

Meyers TA, Heitzman JA, Krebsbach AM, Aufdembrink LM, Hughes R, Bartolomucci A, Townsend D
Journal of molecular and cellular cardiology 2019 Mar;128:51-61

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 (Mouse)

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects ACTN2 protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract 7.5 % SDS-PAGE dilution: 1:1000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Rat tissue extracts (50ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody diluted at 1:5000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] diluted at 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Human heart, using alpha Actinin 2 antibody(10 ug/ml). Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat muscle. alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse muscle. alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat heart, alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. Alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min