A300-573A

antibody from Invitrogen Antibodies

Targeting: UBR5 DD5, EDD, EDD1, HYD, KIAA0896

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-573A

Provider

Invitrogen Antibodies

Product name

EDD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-EDD1 antibody (Product # A300-573A; lot A300-573A-6) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EDD1 antibody A300-573A (lot A300-573A-5) used for IP at 3 µg per reaction. EDD1 was also immunoprecipitated by rabbit anti-EDD1 antibody A303-045A. For blotting immunoprecipitated EDD1, A300-573A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse EDD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EDD1 antibody A300-573A (lot A300-573A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse EDD1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-EDD1 (Product # A300-573A; lot 6) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-EDD1 (Product # A300-573A lot 6) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse EDD1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-EDD1 (Cat. No. A300-573A Lot 4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EDD1 (Cat. No. A300-573A Lot 5) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EDD1 antibody (Product # A300-573A; lot A300-573A-6) used for IP at 6 µg per reaction. EDD1 was also immunoprecipitated by a previous lot of this antibody (lot A300-573A-5). For blotting immunoprecipitated EDD1 (Product # A300-573A) was used at 0.1 µg/mL. Chemiluminescence with an exposure time of 3 minutes.