MAB6036

antibody from R&D Systems

Targeting: CPB2 CPU, PCPB, TAFI

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

MAB6036

Provider

R&D Systems

Product name

Human Carboxypeptidase B2/CPB2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Carboxypeptidase B2/CPB2 in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Carboxypeptidase A1, A4, recombinant mouse (rm) Carboxypeptidase A1, A4, B1, and 5% cross-reactivity with rhCarboxypeptidase B1, B2, and E is observed under reducing conditions. No cross-reactivity with any of these proteins is observed under nonreducing conditions.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q96IY4


Isotype

IgG

Antibody clone number

650801

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Carboxypeptidase B2/CPB2 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Carboxypeptidase B2/CPB2 Monoclonal Antibody (Catalog # MAB6036) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Carboxypeptidase B2/CPB2 at approximately 45 and 50 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.