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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-47089 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPV2 Recombinant Rabbit Monoclonal Antibody (HL1543)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL1543
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRPV2 in unboiled K562 whole cell and membrane extracts (30 µg) separated by 5% SDS-PAGE. Membrane was blotted with TRPV2 Monoclonal antibody (Product # MA5-47089) using a dilution of 1:2,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. The signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRPV2 in K562 cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VRL1 stained by VRL1 monoclonal antibody (Product # MA5-47089) diluted at 1:500. Blue: Fluoroshield with DAPI Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPV2 in paraffin-embedded mouse brain. Samples were incubated in TRPV2 Monoclonal antibody (Product # MA5-47089). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
