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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA309864 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal GLUT10 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal GLUT10 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SLC2A10
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cochlea from embryonic mice was incubated with the GLUT10 primary antibody at 1:500, followed secondary antibody at 1:3,000. Lane 1. 10ug of protein from liver 2. 10ug of protein from cochlea (E18) 3. 10ug of protein from cochlea (P7) 4. 10ug of protein from cochlea (P10) 5. 10ug of protein from cochlea (P14) 6. 10ug of protein from cochlea (P17) 7. 10ug of protein from cochlea (P60) 8. 10ug of protein from cochlea (1 Year)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detetced using anti-GLUT10.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Dissected inner ear tissues was incubated with the GLUT10 antibody at a 1:200 dilution to label the cochlear sections at 4 ? overnight. After washing, the sections were incubated with secondary antibodies (anti-rabbit Cy3, 1:400) at room temperature for 1 hour. Cy3-conjugated secondary antibodies were used to visualize the binding of the primary antibodies. The slides were mounted with fluoromont G and examined under a confocal microscope.
- Validation comment
- IF
