417700

antibody from Invitrogen Antibodies

Targeting: HNF4A HNF4, MODY, MODY1, NR2A1, TCF14

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Gel shift (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

417700

Provider

Invitrogen Antibodies

Product name

Anti-HNF4A Monoclonal Antibody (H1415)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This antibody specifically recognizes human HNF4 alpha 1, 2, 4, 5, 7 ,8 and cross reacts with mouse and rat HNF4 alpha 1, 2, 4, 5, 7 ,8.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

H1415

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

References

Submitted references

Diabetes-linked transcription factor HNF4α regulates metabolism of endogenous methylarginines and β-aminoisobutyric acid by controlling expression of alanine-glyoxylate aminotransferase 2.

Burdin DV, Kolobov AA, Brocker C, Soshnev AA, Samusik N, Demyanov AV, Brilloff S, Jarzebska N, Martens-Lobenhoffer J, Mieth M, Maas R, Bornstein SR, Bode-Böger SM, Gonzalez F, Weiss N, Rodionov RN
Scientific reports 2016 Oct 18;6:35503

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Effects of maternal nutrient restriction, intrauterine growth restriction, and glucocorticoid exposure on phosphoenolpyruvate carboxykinase-1 expression in fetal baboon hepatocytes in vitro.

Li C, Shu ZJ, Lee S, Gupta MB, Jansson T, Nathanielsz PW, Kamat A
Journal of medical primatology 2013 Aug;42(4):211-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-HNF4A Monoclonal Antibody (H1415) (Product # 417700) and a 53kDa band corresponding to HNF4A was observed across all the cell lines tested expect HCT-116 and HeLa which are reported to be negative. Whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), HCT 116 (Lane 2), SW480 (Lane 3), HT-29 (Lane 4), Hep G2 (Lane 5) and HeLa (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).