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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- R35678 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HNF4A Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids RLSKTLVDMDMADY were used as the immunogen for this HNF4A antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the HNF4A antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HepG2 lysate with HNF4A antibody at 0.1ug/ml. Predicted molecular weight ~50/51/45kDa (isoforms 1/2/3).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse small intestine lysate with HNF4A antibody at 0.5ug/ml. Predicted molecular weight ~53/51.5 kDa (long/short form).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human small intestine tissue with HNF4A antibody at 2ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
